Added: Nov 18, 2008

From: mbrpointscom

Duration: 1:8

This video shows how rnai works in the cells.info ref: http://en.wikipedia.org/wiki/RNAi

Channel: People

Tags: bell  bernd  cells  gratzl  interference  kobald  martha  rna  rnai  roland 

Rating: 4.00 (3 ratings)    Views: 8889' favoriteCount='18    Comments: 2

QuantumKitten2 Says:

Nov 18, 2008 - Hmm... is the RISC holoenzyme the same for endogenous miRNAs and transfected siRNAs/shRNAs?

easternblot Says:

Nov 18, 2008 - Yes, it's the same. The difference is earlier: miRNAs are first processed in the nucleus by Drosha - siRNA and shRNA (usually) are not. shRNA are ceaved by Dicer in the cytoplasm. siRNA resembles the product of Dicer. All three (miRNA processed by Drosha/Dicer, shRNA cleaved by Dicer, and siRNA) are about the same shape and size at this point and are all recognized by the RISC complex.